Assay of leukotriene B4 production in stimulated whole blood

The regulation of CD11b integrin levels on human blood leukocytes and leukotriene B4-stimulated skin by a specific leukotriene B4 receptor antagonist (LY293111).

CD11b is part of the beta2-integrin Mac-1 and plays an important role in neutrophil adhesion. Leukotriene B4 (LTB4) is an active upregulator of neutrophil CD11b-expression, acts as a potent chemoattractant to neutrophils and is also known to upmodulate epidermal proliferation. We performed a placebo-controlled study on LY293111, an oral LTB4 receptor antagonist. Twenty healthy male volunteers w...

Leukotriene B4 and asthma.

Murine Glomerular Leukotriene B4 Synthesis

Leukotriene (LT) B4 is an important pro-inflammatory autocoid. In order to investigate the potential role of this eicosanoid in renal inflammation, in this study we determined the capability of glomeruli to synthesize this mediator. Glomeruli were able to synthesize LTB4 when provided with exogenous substrate in a dose-dependent fashion in the presence of ionophore A23187. Ionophore, although b...

Enhancement of leukotriene B4 release in stimulated asthmatic neutrophils by platelet activating factor.

BACKGROUND The role of platelet activating factor (PAF) in asthma remains controversial. The priming effect of PAF on leukotriene B4 (LTB4) release, 5-lipoxygenase activity, and intracellular calcium levels in asthmatic neutrophils was examined. METHODS LTB4 and other lipoxygenase metabolites in neutrophils obtained from 17 asthmatic patients and 15 control subjects were measured by reverse p...

Urine leukotriene B4 in familial Mediterranean fever.

OBJECTIVE To determine urinary leukotriene B4 (LTB4) levels and their role in FMF: METHODS Urinary LTB4 levels were studied using a commercial ELISA kit in 12 FMF patients during abdominal attacks, and 20 FMF patients during remission. RESULTS Urinary LTB4 levels in FMF patients during attacks were comparable to those during remission, but higher than normal levels (p = 0.03). CONCLUSIONS...